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fop control plasmid  (Addgene inc)


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    Structured Review

    Addgene inc fop control plasmid
    Compound 15 inhibits Wnt/β‐catenin activity luciferase assay in HEK‐293T <t>cells</t> <t>transfected</t> with M50 Super 8x TOPFlash (TOP) or its mutated version <t>M51</t> Super 8x FOPFlash <t>(FOP)</t> used as negative control. Cells were induced with LiCl (50 mM) and treated with the indicated concentrations of 15 for 24 h. Data are represented as the mean ± SD of three independent experiments, each performed in triplicate. * p < 0.05, ** p < 0.01, *** p < 0.005, ns not significant as determined by analysis of variance (ANOVA).
    Fop Control Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 258 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mutant+control+m51+super+8x+fopflash/M51+Super+8x+FOPFlash+(TOPFlash+mutant)+(Plasmid+%2312457)/pmc12935169-352-13-20
    Average 94 stars, based on 258 article reviews
    fop control plasmid - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "4‐(5‐Chloro‐3‐(3,4,5‐trimethoxybenzoyl)‐1 H ‐indol‐1‐yl)benzenesulfonamide: A Novel Polypharmacology Agent to Target Carbonic Anhydrase IX and XII With Improved Selectivity, Wnt/β‐Catenin Signaling Pathway, and P‐Glycoprotein"

    Article Title: 4‐(5‐Chloro‐3‐(3,4,5‐trimethoxybenzoyl)‐1 H ‐indol‐1‐yl)benzenesulfonamide: A Novel Polypharmacology Agent to Target Carbonic Anhydrase IX and XII With Improved Selectivity, Wnt/β‐Catenin Signaling Pathway, and P‐Glycoprotein

    Journal: Chemmedchem

    doi: 10.1002/cmdc.202500996

    Compound 15 inhibits Wnt/β‐catenin activity luciferase assay in HEK‐293T cells transfected with M50 Super 8x TOPFlash (TOP) or its mutated version M51 Super 8x FOPFlash (FOP) used as negative control. Cells were induced with LiCl (50 mM) and treated with the indicated concentrations of 15 for 24 h. Data are represented as the mean ± SD of three independent experiments, each performed in triplicate. * p < 0.05, ** p < 0.01, *** p < 0.005, ns not significant as determined by analysis of variance (ANOVA).
    Figure Legend Snippet: Compound 15 inhibits Wnt/β‐catenin activity luciferase assay in HEK‐293T cells transfected with M50 Super 8x TOPFlash (TOP) or its mutated version M51 Super 8x FOPFlash (FOP) used as negative control. Cells were induced with LiCl (50 mM) and treated with the indicated concentrations of 15 for 24 h. Data are represented as the mean ± SD of three independent experiments, each performed in triplicate. * p < 0.05, ** p < 0.01, *** p < 0.005, ns not significant as determined by analysis of variance (ANOVA).

    Techniques Used: Activity Assay, Luciferase, Transfection, Negative Control

    Related Articles

    Construct:

    Article Title: β-Catenin inhibition shapes tumor immunity and synergizes with immunotherapy in colorectal cancer
    Article Snippet: Numbers of viable cells were quantified using Cell Counting Kit-8 (CCK-8) (Dojindo, Japan). .. Wnt/β-catenin pathway reporter construct M50 Super 8X TOPflash (Addgene plasmid #12456) or its mutant control M51 Super 8X FOPflash (Addgene plasmid #12457) were kind gifts from Randall Moon.26 Transient plasmid transfection in 293 T cells was performed using jetPRIME transfection reagent (Polyplus Transfection). ..

    Article Title: β-Catenin inhibition shapes tumor immunity and synergizes with immunotherapy in colorectal cancer
    Article Snippet: Numbers of viable cells were quantified using Cell Counting Kit-8 (CCK-8) (Dojindo, Japan). .. Wnt/β-catenin pathway reporter construct M50 Super 8X TOPflash (Addgene plasmid #12456) or its mutant control M51 Super 8X FOPflash (Addgene plasmid #12457) were kind gifts from Randall Moon. .. Transient plasmid transfection in 293 T cells was performed using jetPRIME transfection reagent (Polyplus Transfection).

    Plasmid Preparation:

    Article Title: β-Catenin inhibition shapes tumor immunity and synergizes with immunotherapy in colorectal cancer
    Article Snippet: Numbers of viable cells were quantified using Cell Counting Kit-8 (CCK-8) (Dojindo, Japan). .. Wnt/β-catenin pathway reporter construct M50 Super 8X TOPflash (Addgene plasmid #12456) or its mutant control M51 Super 8X FOPflash (Addgene plasmid #12457) were kind gifts from Randall Moon.26 Transient plasmid transfection in 293 T cells was performed using jetPRIME transfection reagent (Polyplus Transfection). ..

    Article Title: β-Catenin inhibition shapes tumor immunity and synergizes with immunotherapy in colorectal cancer
    Article Snippet: Numbers of viable cells were quantified using Cell Counting Kit-8 (CCK-8) (Dojindo, Japan). .. Wnt/β-catenin pathway reporter construct M50 Super 8X TOPflash (Addgene plasmid #12456) or its mutant control M51 Super 8X FOPflash (Addgene plasmid #12457) were kind gifts from Randall Moon. .. Transient plasmid transfection in 293 T cells was performed using jetPRIME transfection reagent (Polyplus Transfection).

    Mutagenesis:

    Article Title: β-Catenin inhibition shapes tumor immunity and synergizes with immunotherapy in colorectal cancer
    Article Snippet: Numbers of viable cells were quantified using Cell Counting Kit-8 (CCK-8) (Dojindo, Japan). .. Wnt/β-catenin pathway reporter construct M50 Super 8X TOPflash (Addgene plasmid #12456) or its mutant control M51 Super 8X FOPflash (Addgene plasmid #12457) were kind gifts from Randall Moon.26 Transient plasmid transfection in 293 T cells was performed using jetPRIME transfection reagent (Polyplus Transfection). ..

    Article Title: β-Catenin inhibition shapes tumor immunity and synergizes with immunotherapy in colorectal cancer
    Article Snippet: Numbers of viable cells were quantified using Cell Counting Kit-8 (CCK-8) (Dojindo, Japan). .. Wnt/β-catenin pathway reporter construct M50 Super 8X TOPflash (Addgene plasmid #12456) or its mutant control M51 Super 8X FOPflash (Addgene plasmid #12457) were kind gifts from Randall Moon. .. Transient plasmid transfection in 293 T cells was performed using jetPRIME transfection reagent (Polyplus Transfection).

    Control:

    Article Title: β-Catenin inhibition shapes tumor immunity and synergizes with immunotherapy in colorectal cancer
    Article Snippet: Numbers of viable cells were quantified using Cell Counting Kit-8 (CCK-8) (Dojindo, Japan). .. Wnt/β-catenin pathway reporter construct M50 Super 8X TOPflash (Addgene plasmid #12456) or its mutant control M51 Super 8X FOPflash (Addgene plasmid #12457) were kind gifts from Randall Moon.26 Transient plasmid transfection in 293 T cells was performed using jetPRIME transfection reagent (Polyplus Transfection). ..

    Article Title: β-Catenin inhibition shapes tumor immunity and synergizes with immunotherapy in colorectal cancer
    Article Snippet: Numbers of viable cells were quantified using Cell Counting Kit-8 (CCK-8) (Dojindo, Japan). .. Wnt/β-catenin pathway reporter construct M50 Super 8X TOPflash (Addgene plasmid #12456) or its mutant control M51 Super 8X FOPflash (Addgene plasmid #12457) were kind gifts from Randall Moon. .. Transient plasmid transfection in 293 T cells was performed using jetPRIME transfection reagent (Polyplus Transfection).

    Transfection:

    Article Title: β-Catenin inhibition shapes tumor immunity and synergizes with immunotherapy in colorectal cancer
    Article Snippet: Numbers of viable cells were quantified using Cell Counting Kit-8 (CCK-8) (Dojindo, Japan). .. Wnt/β-catenin pathway reporter construct M50 Super 8X TOPflash (Addgene plasmid #12456) or its mutant control M51 Super 8X FOPflash (Addgene plasmid #12457) were kind gifts from Randall Moon.26 Transient plasmid transfection in 293 T cells was performed using jetPRIME transfection reagent (Polyplus Transfection). ..



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    Addgene inc fop control plasmid
    Compound 15 inhibits Wnt/β‐catenin activity luciferase assay in HEK‐293T <t>cells</t> <t>transfected</t> with M50 Super 8x TOPFlash (TOP) or its mutated version <t>M51</t> Super 8x FOPFlash <t>(FOP)</t> used as negative control. Cells were induced with LiCl (50 mM) and treated with the indicated concentrations of 15 for 24 h. Data are represented as the mean ± SD of three independent experiments, each performed in triplicate. * p < 0.05, ** p < 0.01, *** p < 0.005, ns not significant as determined by analysis of variance (ANOVA).
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    Addgene inc control m51 super 8× fopflash plasmid
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    Addgene inc negative control m51 super 8x fopflash
    Obatoclax antagonizes WNT/β-catenin signaling. ( A ) Obatoclax decreases β-catenin-mediated TCF/LEF-dependent transcriptional activity. CRC cell lines were transiently transfected with M50 Super <t>8x</t> TOPFlash (TOPflash), a luciferase reporter plasmid for the transcriptional activity of TCF/LEF, followed by 200 nM of Obatoclax treatment and ensuing luciferase activity assay. <t>M51</t> Super 8x <t>FOPFlash</t> (FOPflash), a TOPFlash mutant, was used as a negative control. ***: p < 0.001. ( B ) Obatoclax downregulates WNT/β-catenin target genes c-MYC and cyclin D1. DLD-1 and HCT 116 cells were treated with Obatoclax (0, 100, 200 nM), followed by immunoblotting for the levels of c-MYC and cyclin D1. GAPDH was used as a control for equal loading.
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    Image Search Results


    Compound 15 inhibits Wnt/β‐catenin activity luciferase assay in HEK‐293T cells transfected with M50 Super 8x TOPFlash (TOP) or its mutated version M51 Super 8x FOPFlash (FOP) used as negative control. Cells were induced with LiCl (50 mM) and treated with the indicated concentrations of 15 for 24 h. Data are represented as the mean ± SD of three independent experiments, each performed in triplicate. * p < 0.05, ** p < 0.01, *** p < 0.005, ns not significant as determined by analysis of variance (ANOVA).

    Journal: Chemmedchem

    Article Title: 4‐(5‐Chloro‐3‐(3,4,5‐trimethoxybenzoyl)‐1 H ‐indol‐1‐yl)benzenesulfonamide: A Novel Polypharmacology Agent to Target Carbonic Anhydrase IX and XII With Improved Selectivity, Wnt/β‐Catenin Signaling Pathway, and P‐Glycoprotein

    doi: 10.1002/cmdc.202500996

    Figure Lengend Snippet: Compound 15 inhibits Wnt/β‐catenin activity luciferase assay in HEK‐293T cells transfected with M50 Super 8x TOPFlash (TOP) or its mutated version M51 Super 8x FOPFlash (FOP) used as negative control. Cells were induced with LiCl (50 mM) and treated with the indicated concentrations of 15 for 24 h. Data are represented as the mean ± SD of three independent experiments, each performed in triplicate. * p < 0.05, ** p < 0.01, *** p < 0.005, ns not significant as determined by analysis of variance (ANOVA).

    Article Snippet: HEK293T cells were transfected with M50 Super 8x TOPFlash (Addgene #12456) or the FOP control plasmid (M51 Super 8x FOPFlash Addgene #12457) in combination with TK Renilla (Promega #E2241) using DreamFect Gold (OZ Biosciences #DG80500) according to the manufacturer's instructions.

    Techniques: Activity Assay, Luciferase, Transfection, Negative Control

    Obatoclax antagonizes WNT/β-catenin signaling. ( A ) Obatoclax decreases β-catenin-mediated TCF/LEF-dependent transcriptional activity. CRC cell lines were transiently transfected with M50 Super 8x TOPFlash (TOPflash), a luciferase reporter plasmid for the transcriptional activity of TCF/LEF, followed by 200 nM of Obatoclax treatment and ensuing luciferase activity assay. M51 Super 8x FOPFlash (FOPflash), a TOPFlash mutant, was used as a negative control. ***: p < 0.001. ( B ) Obatoclax downregulates WNT/β-catenin target genes c-MYC and cyclin D1. DLD-1 and HCT 116 cells were treated with Obatoclax (0, 100, 200 nM), followed by immunoblotting for the levels of c-MYC and cyclin D1. GAPDH was used as a control for equal loading.

    Journal: International Journal of Molecular Sciences

    Article Title: Obatoclax, a Pan-BCL-2 Inhibitor, Downregulates Survivin to Induce Apoptosis in Human Colorectal Carcinoma Cells Via Suppressing WNT/β-catenin Signaling

    doi: 10.3390/ijms21051773

    Figure Lengend Snippet: Obatoclax antagonizes WNT/β-catenin signaling. ( A ) Obatoclax decreases β-catenin-mediated TCF/LEF-dependent transcriptional activity. CRC cell lines were transiently transfected with M50 Super 8x TOPFlash (TOPflash), a luciferase reporter plasmid for the transcriptional activity of TCF/LEF, followed by 200 nM of Obatoclax treatment and ensuing luciferase activity assay. M51 Super 8x FOPFlash (FOPflash), a TOPFlash mutant, was used as a negative control. ***: p < 0.001. ( B ) Obatoclax downregulates WNT/β-catenin target genes c-MYC and cyclin D1. DLD-1 and HCT 116 cells were treated with Obatoclax (0, 100, 200 nM), followed by immunoblotting for the levels of c-MYC and cyclin D1. GAPDH was used as a control for equal loading.

    Article Snippet: M50 Super 8x TOPFlash (Addgene plasmid # 12456; http://n2t.net/addgene:12456 ; RRID:Addgene_12456), the luciferase reporter vector for TCF/LEF-mediated transcriptional activity, and its negative control M51 Super 8x FOPFlash (TOPFlash mutant) (Addgene plasmid # 12457; http://n2t.net/addgene:12457 ; RRID:Addgene_12457) were gifts from Professor Randall Moon.

    Techniques: Activity Assay, Transfection, Luciferase, Plasmid Preparation, Mutagenesis, Negative Control, Western Blot, Control